發(fā)布者：抗性基因網(wǎng) 時間：2023-06-14 瀏覽量：1503
摘要
出身背景
不同畜牧生產(chǎn)系統(tǒng)中的畜牧業(yè)實踐和牲畜與野生動物相互作用的增加被認為是干旱和半干旱地區(qū)抗微生物耐藥性（AMR）的主要驅(qū)動因素。盡管在過去十年中，駱駝的數(shù)量增加了十倍，再加上駱駝產(chǎn)品的廣泛使用，但缺乏關(guān)于這些生產(chǎn)系統(tǒng)中產(chǎn)生β-內(nèi)酰胺酶的大腸桿菌（E.coli）的全面信息。
目標
我們的研究試圖建立AMR譜，并從肯尼亞北部駱駝群的糞便樣本中分離出新出現(xiàn)的產(chǎn)β-內(nèi)酰胺酶的大腸桿菌，并對其進行鑒定。
方法
采用紙片擴散法建立了大腸桿菌分離株的耐藥性譜，并對β-內(nèi)酰胺酶（bla）基因進行了PCR產(chǎn)物測序，進行了系統(tǒng)發(fā)育分組和遺傳多樣性評估。
后果
我們發(fā)現(xiàn)，在回收的大腸桿菌分離株（n=123）中，對頭孢克洛的耐藥性最高，為28.5%，其次是頭孢噻肟，為16.3%，氨芐青霉素，為9.7%。此外，在3.3%的總樣本中檢測到攜帶blaCTX-M-15或blaCTX-M-27基因的產(chǎn)超廣譜β-內(nèi)酰胺酶（ESBL）的大腸桿菌，并且與系統(tǒng)發(fā)育組B1、B2和D相關(guān)。檢測到非ESBL blaTEM基因的多個變體，其中大多數(shù)是blaTEM-1和blaTEM-116基因。
結(jié)論
這項研究的結(jié)果揭示了在具有多藥耐藥性表型的大腸桿菌分離株中，ESBL和非ESBL編碼基因變異的發(fā)生率增加。這項研究強調(diào)了擴大“一個健康”方法的必要性，以了解AMR的傳播動態(tài)、AMR發(fā)展的驅(qū)動因素，以及ASAL內(nèi)駱駝生產(chǎn)系統(tǒng)中抗菌藥物管理的適當實踐。
Abstract
Background
Animal husbandry practices in different livestock production systems and increased livestock–wildlife interactions are thought to be primary drivers of antimicrobial resistance (AMR) in Arid and Semi-Arid Lands (ASALs). Despite a tenfold increase in the camel population within the last decade, paired with widespread use of camel products, there is a lack of comprehensive information concerning beta-lactamase-producing Escherichia coli (E. coli) within these production systems.
Objectives
Our study sought to establish an AMR profile and to identify and characterise emerging beta-lactamase-producing E. coli isolated from faecal samples obtained from camel herds in Northern Kenya.
Methods
The antimicrobial susceptibility profiles of E. coli isolates were established using the disk diffusion method, with beta-lactamase (bla) gene PCR product sequencing performed for phylogenetic grouping and genetic diversity assessments.
Results
Here we show, among the recovered E. coli isolates (n = 123), the highest level of resistance was observed for cefaclor at 28.5% of isolates, followed by cefotaxime at 16.3% and ampicillin at 9.7%. Moreover, extended-spectrum beta-lactamase (ESBL)-producing E. coli harbouring the blaCTX-M-15 or blaCTX-M-27 genes were detected in 3.3% of total samples, and are associated with phylogenetic groups B1, B2 and D. Multiple variants of non-ESBL blaTEM genes were detected, the majority of which were the blaTEM-1 and blaTEM-116 genes.
Conclusions
Findings from this study shed light on the increased occurrence of ESBL- and non-ESBL-encoding gene variants in E. coli isolates with demonstrated multidrug resistant phenotypes. This study highlights the need for an expanded One Health approach to understanding AMR transmission dynamics, drivers of AMR development, and appropriate practices for antimicrobial stewardship in camel production systems within ASALs.
https://onlinelibrary.wiley.com/doi/full/10.1002/vms3.1090