發(fā)布者：抗性基因網(wǎng) 時間：2023-06-14 瀏覽量：682
摘要
? ? ? 以生物膜為核心的純移動床生物膜反應器（純MBBR）是一種可行的抗生素處理工藝?；前粪奏ぃ⊿DZ）對純MBBR的影響尚待深入研究。本研究檢測了在純MBBR中生物膜暴露于不同劑量的SDZ（0、1、3、5、7和10mg/L）的反應。在連續(xù)流條件下，純MBBR具有較高的SDZ去除率，但SDZ顯著抑制了COD和TN的去除。然而，1-3 mg/L的SDZ可以提高NH4+-N和TP的去除率。生物量分析和多組學測序數(shù)據(jù)證實了這些觀察結(jié)果。SDZ加速了好氧生物膜的脫落和更新，從而縮短了固體的停留時間。SDZ強烈改變了生物膜群落，自養(yǎng)生物的活性受到SDZ的抑制小于異養(yǎng)生物。同時，SDZ并沒有改變生物膜的主要氮代謝機制，同時破壞了反硝化基因與硝化基因的比例。SDZ有效地促進了amoCAB和hao的基因表達，但抑制了norBC的表達?？剐曰騍ul1-3豐度的增加是生物膜能夠適應高濃度SDZ的主要原因?？偟膩碚f，當SDZ低于3mg/L時，純MBBR可以很好地操作。這項研究為純MBBR處理抗生素廢水提供了新的見解。
Abstract
Pure Moving bed biofilm reactor (pure MBBR) with biofilm as biomass core is a viable process for treating antibiotics. The influences of sulfadiazine (SDZ) on pure MBBR have yet to be investigated in depth. This study examined the responses of biofilm exposure to different doses of SDZ (0, 1, 3, 5, 7 and 10 mg/L) in the pure MBBR. Under continuous-flow conditions, pure MBBR held a high SDZ removal rate, but SDZ significantly inhibited COD and TN removal. However, 1–3 mg/L SDZ could enhance NH4+-N and TP removal. And these observations were confirmed by biomass analysis and multi-omics sequencing data. SDZ accelerated the sloughing and renewal of aerobic biofilm, thus shortening the solids residence time. The biofilm communities were strongly shifted by SDZ and the activity of autotrophs was less inhibited by SDZ than that of heterotrophs. Meanwhile, SDZ did not alter the biofilm's primary nitrogen metabolism mechanism, while disrupting the ratio of denitrification genes to nitrification genes. SDZ effectively promoted the gene expression of amoCAB and hao, but restrained norBC. The increased abundance of resistance genes Sul1–3 was a major reason for the biofilm could adapt to the high concentration of SDZ. Overall, the pure MBBR could operate well when SDZ was below 3 mg/L. This study provided new insight into antibiotic wastewater treatment through pure MBBR.
https://www.sciencedirect.com/science/article/abs/pii/S2214714422008716