發(fā)布者：抗性基因網(wǎng) 時(shí)間：2023-06-14 瀏覽量：537
摘要
? ? ? 購(gòu)物中心為微生物種群提供了各種生態(tài)位，有可能成為公共衛(wèi)生問(wèn)題微生物傳播的來(lái)源和蓄水池。然而，關(guān)于微生物組和人類(lèi)病原體在商場(chǎng)中的分布的知識(shí)在很大程度上是未知的。在這里，我們檢測(cè)了來(lái)自購(gòu)物中心地板和自動(dòng)扶梯表面以及鄰近道路灰塵和綠地土壤的微生物群落動(dòng)態(tài)和潛在病原體的基因型。微生物群落的分布模式主要受棲息地和季節(jié)的驅(qū)動(dòng)。室內(nèi)環(huán)境中人類(lèi)相關(guān)微生物群的顯著富集表明，人類(lèi)與表面的相互作用可能是微生物群的另一個(gè)強(qiáng)大驅(qū)動(dòng)因素。中性群落模型表明，微生物群落的組裝是由隨機(jī)過(guò)程強(qiáng)烈驅(qū)動(dòng)的。微生物分類(lèi)特征在環(huán)境分類(lèi)中的獨(dú)特表現(xiàn)表明，不同季節(jié)/棲息地的微生物群落存在一致的差異，以及人為因素對(duì)購(gòu)物中心微生物群落均質(zhì)化的強(qiáng)烈影響。室內(nèi)環(huán)境中攜帶的人類(lèi)病原體濃度高于室外樣本，也攜帶了高比例的抗微生物耐藥性相關(guān)的多藥外排基因和毒力基因。這些發(fā)現(xiàn)增強(qiáng)了對(duì)建筑環(huán)境中微生物組以及人類(lèi)與建筑環(huán)境之間相互作用的理解，為追蹤生物威脅和傳染病以及開(kāi)發(fā)復(fù)雜的預(yù)警系統(tǒng)提供了基礎(chǔ)。
ABSTRACT
Shopping malls offer various niches for microbial populations, potentially serving as sources and reservoirs for the spread of microorganisms of public health concern. However, knowledge about the microbiome and the distribution of human pathogens in malls is largely unknown. Here, we examine the microbial community dynamics and genotypes of potential pathogens from floor and escalator surfaces in shopping malls and adjacent road dusts and greenbelt soils. The distribution pattern of microbial communities is driven primarily by habitats and seasons. A significant enrichment of human-associated microbiota in the indoor environment indicates that human interactions with surfaces might be another strong driver for mall microbiomes. Neutral community models suggest that the microbial community assembly is strongly driven by stochastic processes. Distinct performances of microbial taxonomic signatures for environmental classifications indicate the consistent differences of microbial communities of different seasons/habitats and the strong anthropogenic effect on homogenizing microbial communities of shopping malls. Indoor environments harbored higher concentrations of human pathogens than outdoor samples, also carrying a high proportion of antimicrobial resistance-associated multidrug efflux genes and virulence genes. These findings enhanced the understanding of the microbiome in the built environment and the interactions between humans and the built environment, providing a basis for tracking biothreats and communicable diseases and developing sophisticated early warning systems.
https://journals.asm.org/doi/full/10.1128/msystems.00576-22