發(fā)布者：抗性基因網(wǎng) 時(shí)間：2023-06-14 瀏覽量：2142
摘要
? ? ? 該研究通過(guò)qPCR定量了德國(guó)23個(gè)不同污水處理廠廢水中抗生素抗性基因（ARGs）和兼性致病菌（FPB）的豐度，以及基因組DNA中的一個(gè)移動(dòng)遺傳元件。將12種臨床相關(guān)的ARGs分為常見、中期和罕見的公共廢水遺傳參數(shù)。對(duì)5種針對(duì)大腸桿菌、銅綠假單胞菌、肺炎克雷伯菌、鮑曼不動(dòng)桿菌和腸球菌的FPB進(jìn)行了分類PCR定量。污水處理廠的集水區(qū)不同，僅受醫(yī)院、食品加工公司或住房區(qū)的影響。研究發(fā)現(xiàn)，分析的ARG和FPB的總排放量與污水處理廠的大小無(wú)關(guān)，一個(gè)集群內(nèi)的最大差異為兩個(gè)對(duì)數(shù)單位。最初，定量數(shù)據(jù)評(píng)估顯示ARG類別和污水處理廠集水區(qū)之間沒(méi)有顯著差異。Pearson相關(guān)性方法使更明顯的相關(guān)性變得明顯，其中將每個(gè)單獨(dú)的分類標(biāo)記與每個(gè)ARG靶標(biāo)進(jìn)行比較。在受醫(yī)院廢水影響的污水處理廠廢水中，發(fā)現(xiàn)FPB（即大腸桿菌、肺炎克雷伯菌、銅綠假單胞菌和腸球菌）與罕見耐藥基因（blaNDM-1、vanA）類臨床相關(guān)ARG的相關(guān)性增加。
Abstract
The study quantified the abundances of antibiotic resistance genes (ARGs) and facultative pathogenic bacteria (FPB) as well as one mobile genetic element in genomic DNA via qPCR from 23 different wastewater treatment plant (WWTP) effluents in Germany. 12 clinically relevant ARGs were categorized into frequently, intermediately, and rarely occurring genetic parameters of communal wastewaters. Taxonomic PCR quantifications of five FPB targeting Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumannii, and enterococci were performed. The WWTPs differed in their catchment areas being impacted by hospitals, food processing companies, or housing areas only. The total discharges of the analyzed ARGs and FPB were found to cluster independently of the sizes of the WWTPs with a maximum difference of two log units within one cluster. Initially, quantitative data evaluations revealed no significant difference between ARG categories and WWTP catchment areas. More distinct correlations became obvious with a Pearson correlation approach, where each single taxonomic marker is compared to each ARG target. Here, increased correlation of FPB (i.e. E. coli, K. pneumoniae, P. aeruginosa, and enterococci) with clinically relevant ARGs of the category of rarely occurring resistance genes (blaNDM-1, vanA) was found in WWTP effluents being influenced by hospital wastewaters.
https://www.ifg.kit.edu/7602_7922.php