發(fā)布者：抗性基因網(wǎng) 時(shí)間：2023-06-14 瀏覽量：1712
摘要
背景：這項(xiàng)工作報(bào)告了2007-2020年由“侵襲性A組鏈球菌監(jiān)測計(jì)劃”收集的西班牙侵襲性化膿性鏈球菌的抗微生物耐藥性數(shù)據(jù)。方法：通過測序確定emm分型。通過E試驗(yàn)測定對青霉素、四環(huán)素、紅霉素和克林霉素的敏感性。通過PCR尋找tetM、tetO、msrD、mefA、ermB、ermTR和ermT。使用紅霉素-克林霉素雙圓盤試驗(yàn)檢測大環(huán)內(nèi)酯類耐藥表型（M、cMLSB和iMLSB）。抗性克隆通過其emm型、多位點(diǎn)序列型（ST）、抗性基因型和大環(huán)內(nèi)酯類抗性表型進(jìn)行鑒定。結(jié)果：青霉素的敏感性是普遍的。237/1983個(gè)分離株（12.0%）記錄到四環(huán)素耐藥性（152個(gè)僅攜帶tetM，48個(gè)僅攜帶tetO，33個(gè)同時(shí)攜帶兩者）。在172/1983個(gè)分離株中檢測到紅霉素耐藥性（8.7%）；ermB存在于83例，mefA存在于58例，msrD存在于51例，ermTR存在于46例，ermT存在于36例。78/1983個(gè)分離株中存在克林霉素耐藥性（甲基化酶介導(dǎo)）（3.9%）。鑒定出8個(gè)主要耐藥克隆：2個(gè)僅對四環(huán)素耐藥（emm22/ST46/tetM和emm77/ST63/tetO），3個(gè)僅對紅霉素耐藥（emm4/ST39/mefA-msrD/M、emm12/ST36/mefA-ms rD/M和emm28/ST52/ermB/cMLSB），以及三種四環(huán)素-紅霉素共耐藥（emm11/ST403/ttetM-ermB/cMLSB、emm77/ST63/tetO-ermTR/iMLSB和emm77/ST63/tetM-emtO-ermTR/iMLSB）。結(jié)論：2007年至2020年間，四環(huán)素、紅霉素和克林霉素的耐藥率有所下降?？剐钥寺”壤臅r(shí)間變化決定了抗性率的變化。
Abstract
Background: This work reports on antimicrobial resistance data for invasive Streptococcus pyogenes in Spain, collected by the ‘Surveillance Program for Invasive Group A Streptococcus’, in 2007–2020. Methods: emm typing was determined by sequencing. Susceptibility to penicillin, tetracycline, erythromycin, and clindamycin was determined via the E-test. tetM, tetO, msrD, mefA, ermB, ermTR, and ermT were sought by PCR. Macrolide-resistant phenotypes (M, cMLSB, and iMLSB) were detected using the erythromycin–clindamycin double-disk test. Resistant clones were identified via their emm type, multilocus sequence type (ST), resistance genotype, and macrolide resistance phenotype. Results: Penicillin susceptibility was universal. Tetracycline resistance was recorded for 237/1983 isolates (12.0%) (152 carried only tetM, 48 carried only tetO, and 33 carried both). Erythromycin resistance was detected in 172/1983 isolates (8.7%); ermB was present in 83, mefA in 58, msrD in 51, ermTR in 46, and ermT in 36. Clindamycin resistance (methylase-mediated) was present in 78/1983 isolates (3.9%). Eight main resistant clones were identified: two that were tetracycline-resistant only (emm22/ST46/tetM and emm77/ST63/tetO), three that were erythromycin-resistant only (emm4/ST39/mefA-msrD/M, emm12/ST36/mefA-msrD/M, and emm28/ST52/ermB/cMLSB), and three that were tetracycline–erythromycin co-resistant (emm11/ST403/tetM-ermB/cMLSB, emm77/ST63/tetO-ermTR/iMLSB, and emm77/ST63/tetM-tetO-ermTR/iMLSB). Conclusions: Tetracycline, erythromycin, and clindamycin resistance rates declined between 2007 and 2020. Temporal variations in the proportion of resistant clones determined the change in resistance rates.
https://www.mdpi.com/2079-6382/12/1/99