發(fā)布者：抗性基因網(wǎng) 時(shí)間：2023-06-14 瀏覽量：660
摘要
? ? ? 在本研究中，研究了混凝/絮凝和芬頓工藝的組合作為三級(jí)處理，以產(chǎn)生易于重復(fù)使用的處理水。這兩種工藝的結(jié)合從未應(yīng)用于真正的城市污水消毒。當(dāng)使用120 mg/L的混凝劑（FeCl3）和出水的自然pH（7.14）時(shí)，濁度和腸道細(xì)菌的去除效果最好。當(dāng)使用過氧化氫和添加的鐵濃度分別為100 mg/L和7 mg/L時(shí)，以下芬頓反應(yīng)在25°C下120分鐘后表現(xiàn)出最大的腸道細(xì)菌滅活。在每個(gè)治療步驟前后，評(píng)估抗生素耐藥性（阿莫西林和磺胺甲惡唑）腸道細(xì)菌和總腸道細(xì)菌、腸球菌和異養(yǎng)菌以及抗生素耐藥性基因ARG-（sul1、blaTEM和qnrS）的豐度。在處理后和儲(chǔ)存72小時(shí)后，總的和耐藥的可培養(yǎng)腸道細(xì)菌的值立即低于10 CFU/100 mL，因此達(dá)到了對(duì)大腸桿菌的最嚴(yán)格限制。物理化學(xué)參數(shù)也符合水再利用的既定限制。盡管含有豐富多樣的細(xì)菌群落，但最終儲(chǔ)存的消毒廢水中含有相對(duì)豐度較高的潛在危險(xiǎn)細(xì)菌。這些結(jié)果指出，需要對(duì)處理過的廢水進(jìn)行深入的微生物表征，以評(píng)估其在灌溉中重復(fù)使用的風(fēng)險(xiǎn)。
Abstract
In this study, a combination of coagulation/flocculation and Fenton processes was studied as tertiary treatment in order to generate treated water susceptible to reuse. The combination of both processes has never been applied in disinfection of real urban wastewater. The best removals of turbidity and enterobacteria were achieved when applying a coagulant (FeCl3) dosage of 120 mg/L and the natural pH of the effluent (7.14). The following Fenton reaction presented the maximal enterobacteria inactivation after 120 min at 25 °C, when using hydrogen peroxide and added iron concentrations of 100 mg/L and 7 mg/L, respectively. The abundance of antibiotic resistant (amoxicillin and sulfamethoxazole) enterobacteria and total enterobacteria, enterococci, and heterotrophs, and antibiotic resistance genes - ARG - (sul1, blaTEM and qnrS) was evaluated before and after each step of the treatment. Values below 10 CFU/100 mL were achieved for total and resistant cultivable enterobacteria immediately after treatment and after storage for 72 h, therefore meeting the strictest limit imposed for E. coli. Physico-chemical parameters also met the established limits for water reuse.
Despite harbouring a rich and diverse bacterial community, the final stored disinfected wastewater contained high relative abundance of potentially hazardous bacteria. Such results point out the need of a deep microbiological characterization of treated wastewater to evaluate the risk of its reuse in irrigation.
https://www.sciencedirect.com/science/article/abs/pii/S0013935122023556