發(fā)布者：抗性基因網(wǎng) 時(shí)間：2023-06-14 瀏覽量：670
摘要（Abstrak）
? ? ? 創(chuàng)傷弧菌是一種引起弧菌病的細(xì)菌，對(duì)許多抗菌藥物具有耐藥性。為了全面了解創(chuàng)傷弧菌的耐藥性，選擇了四個(gè)MDR分離株進(jìn)行耐藥性分析。基于平均核苷酸同一性（ANI）和DNA-DNA雜交（DDH），所有分離株都顯示出對(duì)創(chuàng)傷弧菌ATCC 33147的高同一性評(píng)分。同樣，多點(diǎn)序列分型（MLST）顯示，CUVETSS48分離物在更接近兩個(gè)ST（409和570）的地方發(fā)現(xiàn)，而其他分離物（CUVETCC1、CUVETCC2和CUVETSS2）在更接近單個(gè)ST（分別為209、426和372）的地方找到。MIC測(cè)定顯示，所有分離株均對(duì)阿莫西林和硫酸粘菌素產(chǎn)生耐藥性，但只有兩個(gè)分離株（CUVETCC1和CUVETSS48）對(duì)所有測(cè)試的抗菌藥物產(chǎn)生耐藥性。參考耐藥性分析，發(fā)現(xiàn)了多個(gè)抗生素耐藥性基因（ARGs），如QnrVC1、QnrVC7、tetR、tetB和blaCTX-M-55（CUVETCC1）；tet（59）、sul2和QnrVC5（CUVETSS48）。此外，在gyrA的QRDR區(qū)域內(nèi)發(fā)現(xiàn)了一個(gè)突變；S83I（CUVETCC1、CUVETCC2和CUVETSS48），gyrB；K87N（CUVETCC2、SS2和SS48）和parC；S80Y（CUVETCC1和CUVETCC2）?？傊?，本研究首次證明創(chuàng)傷弧菌分離株中存在blaCTX-M-55、QnrVC5抗微生物抗性基因，以及gyrA、gyrB和parC基因突變（位于83、87和80位）。多種ARGs的存在可能有助于創(chuàng)傷弧菌的MDR表型。
Abstract (Abstrak)
Vibrio vulnificus is a bacterium that causes vibriosis and exhibits resistance to many antimicrobials. To comprehensively understand V. vulnificus resistance, four MDRs isolates were selected for resistome analysis. Based on the Average Nucleotide Identity (ANI) and DNA-DNA hybridization (DDH), all isolates showed a high identity score to the V. vulnificus ATCC 33147. Likewise, multilocus sequence typing (MLST) showed that CUVETSS48 isolate was founded closer to two STs (409 and 570), while the others (CUVETCC1, CUVETCC2, and CUVETSS2) were founded closer to a single ST (209, 426, and 372, respectively). MIC determination showed that all isolates were resistant to Amoxicillin and Colistin sulfate, but only two isolates (CUVETCC1 and CUVETSS48) were resistant to all tested antimicrobials. Referring to resistome analysis, multiple antibiotic resistance genes (ARGs) were found, such as QnrVC1, QnrVC7, tetR, tetB, and blaCTX-M-55 (CUVETCC1); tet(59), sul2, and QnrVC5 (CUVETSS48). Moreover, a mutation was founded within the QRDR region of gyrA; S83I (CUVETCC1, CUVETCC2, and CUVETSS48), gyrB; K87N (CUVETCC2, SS2, and SS48), and parC; S80Y (CUVETCC1 and CUVETCC2). In conclusion, this study showed the first evidence for the presence of blaCTX-M-55, QnrVC5 anti- microbial resistance genes, as well as mutations in gyrA, gyrB, and parC genes (at positions 83, 87, and 80) among V. vulnificus isolates. The presence of multiple ARGs may contribute to the MDR phenotype of V. vulnificus.
http://repository.unhas.ac.id/id/eprint/26141/